Search results for "genetic characterization"
showing 8 items of 8 documents
Genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels
2021
AbstractAlmond is appreciated for its nutraceutical value and for the aromatic profile of the kernels. In this work, an almond collection composed of 96 Sicilian accessions complemented with 10 widely cultivated cultivars was phenotyped for the production of volatile organic compounds using a proton-transfer time-of-flight mass spectrometer and genotyped using the Illumina Infinium®18 K Peach SNP array. The profiling of the aroma was carried out on fresh and roasted kernels enabling the detection of 150 mass peaks. Sixty eight, for the most related with sulfur compounds, furan containing compounds, and aldehydes formed by Strecker degradation, significantly increased during roasting, while …
The Girgentana Goat Breed: A Zootechnical Overview on Genetics, Nutrition and Dairy Production Aspects
2017
In recent years, there has been a great interest in recovering and preserving local livestock breeds. An interesting situation is represented by the Girgentana goat, an ancient local breed reared in Sicily. Over recent years, this breed has become almost extinct, in part as a consequence of the marked decrease in fresh goat milk consumption. On the basis of these considerations, several studies on its genetic structure and management aspects have been conducted in order to protect the Girgentana goat from the risk of extinction and recover its genetic and economic value. In this context, information on genetics, nutrition and dairy production aspects may have a crucial role in the improveme…
Population genetics of cucumber mosaic virus infecting medicinal, aromatic and ornamental plants from northern Italy
2012
An epidemiological survey carried at the Herb Garden of Casola Valsenio (Emilia Romagna region; Northern Italy) from 2006 to 2009 resulted in finding Cucumber mosaic virus (CMV) as the infectious agent of several ornamental, medicinal and aromatic plants. All CMV-infected plant species showed leaf symptoms followed, in some cases, by flower colour-breaking and stunting. Genetic analysis consisted in the characterization of movement protein gene of each CMV-isolate by single strand polymorphism analysis and sequencing. Phylogenetic trees identified only two isolates as member of subgroup II, while all other isolates were part of subgroup IA. This survey confirms an unexpected increment of CM…
Genetic Diversity of Major Olive Varieties from Southern Tunisia
2013
Considering the importance of olive-growing in Tunisia, microsatellite (SSR) analysis was used to study the genetic variation among twenty olive accessions from southern Tunisia. This set of olive microsatellites showed potential utility for genetic studies and it could contribute to the development of strategies for Tunisian germplasm conservation and breeding. Unweighted pair group method cluster analysis was performed and cultivars separated in three main groups. Five polymorphic simple sequence repeats (SSR) loci were employed and they revealed 38 alleles with a mean number of 7.6 alleles per locus. Genetic variability was wide as indicated by high values of both observed heterozygosity…
Genetic characterization of the Mascaruna goat, a Sicilian autochthonous population, using molecular markers
2013
The aim of this work was to characterize a Sicilian autochthonous goat population using microsatellite markers and genetic polymorphisms at the casein genes. In order to investigate the genetic structure of the Mascaruna goat, a total of 60 (20 Girgentana, 20 mixed populations, and 20 Mascaruna) individuals were analyzed, using a panel of 18 microsatellite markers. Moreover, the Mascaruna goats were genotyped at casein loci using several molecular techniques. Based on the genetic structure at casein genes, the Mascaruna goat was similar to most goat breeds from the Mediterranean area, which are characterized by the predominance of strong alleles. The low value of genetic differentiation amo…
New records of Lepidochelys olivacea (Eschscholtz, 1829) (Testudines, Cheloniidae) provide evidence that Uruguayan waters are the southernmost limit …
2017
We report 8 new records of Lepidochelys olivacea marine turtle in the Uruguayan waters, indicating this area as the southernmost limit of distribution for this species in the western Atlantic Ocean. In addition, 1 specimen was subjected to genetic analysis, revealing its population origin in the western Atlantic nesting colonies (Surinam, French Guiana, and Brazil). This report represents an update of the distribution of L. olivacea in the southwestern Atlantic and provides insight into the morphological and genetic characterization of the species at temperate waters.
Molecular markers reflect differentiation of Fusarium oxysporum forma speciales on tomato and forma on eggplant
2013
Selective pressure induces pathogens to change their method of infection and, sometimes, causes species to become infectious. Pathogenic fungi must differentiate different morphological and physiological properties during the process of host specialization in their life cycle. In the present study, we conducted a genetic investigation and compared similarities within a generation of Fusarium oxysporum forma speciales (f. sp.) infecting tomato and forma (f.) infecting eggplants using selected ISSR and RAPD markers, two horticultural commodities belonging to the same taxon of the Solanaceae. Interestingly, genetic data showed that fungi belonging to F. oxysporum f. sp. infecting tomato have a…
Genotyping of bovine diarrhea virus (BVDV) isolated from cattle in Sicily
2011
Bovine Viral Diarrhoea - Mucosal Disease (BVD-MD) is a widely spread infectious disease that cause economically important losses in farms. Several epidemiological studies indicate a high genetic heterogeneity among Bovine Viral Diarrhoea Virus (BVDV) strains circulating in Italy. The aim of this study was to investigate the genotypes of BVDV in Sicily, a region in the South of Italy. For this purpose, 17 BVDV strains collected from cattle breed in Sicily between 2005 and 2008 were genetically typed by sequencing of 5'-untraslated region (5’-UTR) of the viral genome. In this study, phylogenetic analysis showed that all 17 examined strains were clustered within the BVDV genotype 1. Particular…